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KMID : 0381920060360040291
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Korean Journal of Microscopy
2006 Volume.36 No. 4 p.291 ~ p.297
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Ultrastructural Changes of Endoplasmic Reticulum on Hepatocytes by Cyclohexane Injection in Alcohol-pretreated Rats
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Kim Byung-Ryul
Yoon Chong-Guk
Cho Hyun-Gug
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Abstract
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To evaluate the effects of ingestion of alcoholic drinks on the toxicities of industrial compounds, cyclohexane (CH) was intraperitoneally administrated to rats (1.56 g/kg body weight), which had been ingested 15% ethanol for up to 6 weeks, 4 times by once a day and every other day. Following the last treatment of ethanol or CH, blood and liver tissues were collected after 4 hours prior to sacrifice of animals. By the injection of CH, liver weight (% of body weight) and xanthine oxidase activity in serum were increased, and glucose-6-phasphatase (G6P) activity in liver was decreased compared to them of control group. The activities of CH metabolizing enzymes, such as cytochrome P450 dependent aniline hydroxylase (CYPdAH) and alcohol dehydrogenase (ADH), were significantly increased by injection of CH, and those activities were the highest in CH-injected group after pretreated with alcohol. Ultrastructurally, both of alcohol treatment and
CH injection induced transforming into the smooth-endoplasmic reticulum from rough-endoplasmic reticulum, the those rate was the highest in case of CH-injection after pretreated with alcohol. From these results, it is suggested that alcohol intake on a level without alcoholic degeneration of hepatocytes could enhance the CH metabolism of liver.
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KEYWORD
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Alcohol, Cyclohexane, Endoplasmic reticulum
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